f88-4 cys6 16-mer cyclic
f88-4 cys6 cyclic peptide phage display library (16-mer)
The f88-4 cys6 cyclic 16-mer peptide phage display library is built from the f88-4 filamentous phage vector ( AF218363.1 ) by replacing a synthetic 21 bp stuffer sequence with frame-restoring 48 bp random oligo nucleotides. The resulting phage particles express the wild-type gene VIII and an artificial gene rVIII. The 16-mer foreign peptide insert is expressed on rVIII. The expressed peptides contains cysteines at positions 5 and 12 (XXXXCXXXXXXCXXXX) constraining the peptide in a cyclic conformation under oxidative conditions. The f88-4 phage display libraries maintain E. coli infectivity and do not require a helper phage for assembly and release of virions.
| Product | Peptide phage display library |
|---|---|
| Cat. Number | COPD0005 |
| Type | pVIII |
| Format | 16-mer cyclic |
| Diversity | 10^9 |
| Clone copies | >100 |
| Excluded codons | Stop and cysteine |
| Elution | Disruption of non-covalent interactions |
| Bacterial resistance | Tetracycline (20 μg/mL) |
| Growth strains | K91BluKan |
| Reference | Scott JK et al. Science. 1990 Jul 27;249(4967):386-90. doi: 10.1126/science. |
| Price | $450.00 |
| SnapGene | Image | Full sequence |
Please Cite
The original f88-4 15-mer peptide phage display library was created by Dr. George Smith at the University of Missouri and is distributed by Cell Origins (#COPD0004).
References section:
Scott JK, Smith GP. Searching for peptide ligands with an epitope library. Science. 1990 Jul 27;249(4967):386-90. doi: 10.1126/science.1696028.