f3TR1
Type 3 trypsin release phage display vector f3TR1
The f3TR1 phage display vector is based on phage display vector fUSE5 ( AF218364.1 ). A 14 bp frameshifting stuffer must be replaced with a frame-restoring peptide insert to express pIII and enable E. coli infectivity. The displayed peptide is fused to the N-terminus of pIII through a trypsin-recognition sequence. The fUSE5 vector can be used to create new phage display peptide libraries by replacing the 14 bp stuffer with random oligonucleotides.
| Product | Bacteriophage display vector |
|---|---|
| Cat. Number | COV0002 |
| Genbank | HM355479.1 |
| Sequence | >HM355479.1 |
| Size | 8958 bp (dsDNA) |
| Insert | 14 bp stuffer |
| Bacterial resistance | Tetracycline (20 μg/mL) |
| Growth strains | K91BluKan |
| Reference | Thomas WD et al. Anal Biochem. 2010. 407(2):237-40. |
| Price | $195.00 |
| SnapGene | Image | Full sequence |
Please Cite
The f3TR1 vector was created by Dr. George Smith at the University of Missouri and is distributed by Cell Origins (#COV0002).
References section:
Thomas WD, Golomb M, Smith GP. Corruption of phage display libraries by target-unrelated clones: diagnosis and countermeasures. Anal Biochem. 2010 Dec 15;407(2):237-40. doi: 10.1016/j.ab.2010.07.037.