f3TR1 15-mer cyclic
f3TR1 linear peptide phage display library (15-mer)
The f3TR1 15-mer peptide phage display library is built from the f3TR1 filamentous phage vector ( HM355479.1 ) by replacing a synthetic 14 bp stuffer sequence with frame-restoring 45 bp random oligo nucleotides. The 15-mer foreign peptide insert is expressed on pIII as a fusion to the N-terminus of pIII through a trypsin-recognition sequence. The f3TR1 phage display library maintains E. coli infectivity and does not require a helper phage for assembly and release of virions.
| Product | Peptide phage display library |
|---|---|
| Cat. Number | COPD0002 |
| Type | pIII |
| Format | 15-mer linear |
| Diversity | 1.91x10^10 |
| Clone copies | >100 |
| Excluded codons | Stop and cysteine |
| Elution | Trypsin digestion, disruption of non-covalent interactions |
| Bacterial resistance | Tetracycline (20 μg/mL) |
| Growth strains | K91BluKan |
| Reference | Scott JK et al. Science. 1990 Jul 27;249(4967):386-90. doi: 10.1126/science. |
| Price | $4750.00 |
| Certificate of Analysis | COA-COPD0002 |
| Image |
Please Cite
The f3TR1 vector was created by Dr. George Smith at the University of Missouri and the f3TR1 linear 15-mer library was created by Cell Origins (#COPD0002).
References section:
Thomas WD, Golomb M, Smith GP. Corruption of phage display libraries by target-unrelated clones: diagnosis and countermeasures. Anal Biochem. 2010 Dec 15;407(2):237-40. doi: 10.1016/j.ab.2010.07.037.